Molecular biology protocols assessment in the Biochemistry subject of the National School of Dentistry, National University of Mexico (UNAM)

Gloria Gutiérrez Venegas, Sergio Sánchez García, Alejandro Golzarri Moreno, Adrián Ramírez Peralta, Cristina Hernández Bermúdez


For over a decade, experimental teaching of Biochemistry as a subject has been reinforced as part of the School of Dentistry, National University of Mexico (UNAM) curriculum. This aim has been achieved with the help of laboratory practices. The project of strengthening biochemistry teaching, entailed the purpose of achieving, for the student, the comprehension of biochemistry and molecular biology basic concepts and thus improving their professional skills. With this purpose in mind, three molecular biology experimental protocols have been introduced in the scholastic cycle 2011-2012. Objective: To analyze in the 2011-2012 generation, the opinion survey sustained on molecular biology protocols. Materials and methods: In an blind and voluntary basis, an opinion survey was applied to fi rst year students of the National School of Dentistry. In this survey, six aspects of molecular biology protocols were assessed. The six aspects were: learning, participation, planning, group interaction, evaluation and infrastructure. Standard deviation means were obtained (± SD) in order to assess instrument reliability. Internal consistency of the survey and evaluation percentage of each reactive were equally obtained. To achieve this aim, the SPSS Windows version 12 was used. Averages of different categories were as follows: learning 71.1% (± 12.5), evaluation: 78.6% (± 17.1); infrastructure: 81.8% (± 17.9%). Global assessment revealed a 78.1 ± 10.3. Internal consistency of the survey presented a Crombach alpha of global 0.889. Survey of analysis results suggested that contemporary training for dentistry students must combine the didactic experience of laboratory work along with classroom activities. Infrastructure must nevertheless be potentiated as well as the student’s skills to interpret results.

Palabras clave

Biochemistry, molecular biochemistry, survey, protocols’ assessment.

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