Citotoxicity assessment of different endodontic-use sealing cements in gingival fi broblast cultures

Abstract

The aim of the present study was the in vitro evaluation of theresponse, within 96 hours, of gingival fi broblast cultures with respectto different endodontic sealers. Results obtained at time intervalsof 0, 1, 2, 3, 6, 24, 48, 72 and 96 hours were used to determinesealers´ cytotoxicity. Gingival fi broblasts cultures without root canalsealer and with Sealapex were used as negative and positivecontrols respectively. Results were compared with negative controlsand statistically analyzed with t Dunnett test (p < 0.05). Assessedsealing cements were: ProRoot MTA, grey and white, CPM, MTAAngelus, Sealapex and GuttaFlow. Results showed that eventhough ProRoot MTA (grey and white) MTA Angelus, CPM andGuttaFlow exhibited lower cytotoxic potential than Sealapex, nostatistical signifi cant differences were established.