Evaluación in vitro de la citotoxicidad de tres selladores endodóncicos sobre fibroblastos de ratón

Abstract

Endodotic sealers may cause periapical tissue cytotoxicyty following root canal treatments. In general, in vitro biocompatibility tests evaluate the cytotoxicity of sealers long after they have hardened. Hence, in this study we evaluated the acute effects of three sealers: AH-Plus™, RoekoSealAutomix® (RSA) y Roth- Root using a pore membrane system where the sealers are placed on top just after mixing. Mouse fibroblast cultures were exposed for 6, 12, 24, 36, 48 y 72 hours to the sealers described above. After each period, the morphology and viability of fibroblasts were evaluated. There were no differences in morphology between cells exposed to RSA and control conditions. Fibroblasts exposed to the other two sealers detached from the culture dish, lost their flame-like morphology and appeared rounded. The number of cells in cultures exposed to RSA was similar to that of the control cultures. In contrast, fibroblast exposed to the other two sealers died after 6 hours of culture. In conclusion, because RSA essentially showed no cytotoxicyty, it appears that silicon-based sealers are the best options for treating root canals.