In Vitro Adhesion of Candida albicans in Three Different Tissue Conditioners Used in Prosthodontics

Abstract

Background: Tissue conditioners are materials used on the surface of denture bases for 4 to 7 days. These materials have an irregular surface, and if improperly used, there is the possibility of adherence of some microorganisms such as Candida albicans which could elicit Oral Candidiasis.

Objective: To quantify and analyze three tissue conditioners with the aid of an Optical Microscope (OM) as well as a Scanning Electron Microscope (SEM) at three inoculation phases (days)

Study:36 study samples were prepared through inoculation of in vitro  Candida albicans at 24, 72 and 168 hour intervals. Samples were assessed through OM and SEM from which photographs were obtained at 500x, 2,000x and 5,000x magnifications.

Results Coe Comfort tissue conditioner presented an even surface with budding yeast adhesion and hyphae; Lynal presented  a 3µ cracked  surface at 72 and 168 hours  with budding yeast; Flexacryl  Presented a 4µ cracked surface at 72 and 168 hours and great amounts of budding yeasts.

Conclusions: Coe Comfort conditioner presents a more regular surface with less Candida albicans adhesion, whereas Flexacryl is the one presenting greater irregularities and Candida albicans adherence  in its surface.